Coding

Part:BBa_K4757013

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-10-03)


AlkS-epS4-156

The wildtype (WT) AlkS transcription factor can be positively regulated with alkanes with a length up to C12. This engineered AlkS with the point mutation V760E was shown to be able to sense alkanes up to C17.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1705
    Illegal EcoRI site found at 1801
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1705
    Illegal EcoRI site found at 1801
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1705
    Illegal EcoRI site found at 1801
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1705
    Illegal EcoRI site found at 1801
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1705
    Illegal EcoRI site found at 1801
    Illegal NgoMIV site found at 219
    Illegal NgoMIV site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None